Prof. Hiroyoshi Takano, Prof. Sunao Katsuki, Prof. Hisato Saitoh

We are going to explore the biological effects of pulsed-power-produced agents such as intense electric fields (more than 100 kV/m) and strong magnetic fields (more than 30 tesla), intense pulsed ultraviolet light, atmospheric pressure plasmas and underwater streamer plasmas, by means of biological analysis. In the last few years, we have found that the agents initiate unique biological processes such as DNA fragmentation induced by high power radio frequency electric fields, morphology change of water bloom due to the exposure to underwater plasmas etc. Our deep interests go to, for example, main receptors of the agents, biological processes, and controllability of the processes in addition to possibility of the deformation of bio molecules such as DNA and protein.

  1. Fukuda, I., Ito, A., Uramoto, M, Saitoh, H., Kawasaki, H., Osada, H., Yoshida, M. (2008) Kerriamycin B inhibits protein SUMOylation. J. Antibiotics, in press.
  2. Izumi, Y., Kuroki, J., Nagafuji, H., Lin, X. and Takano, H. (2009) Effects of antibiotics that inhibit bacterial peptidoglycan synthesis on plastid division in pteridophytes. Cytologia, in press
  3. Nomura, N., Yano, M., Katsuki, S., Akiyama, H., Abe, K., Abe, S-I. (2009) Intracellular DNA Damage Induced by non-thermal, intense narrowband electric fields, IEEE Transactions on Dielectrics and Electrical Insulation, in press.
  4. Choi, J., Matsuo, K., Yoshida, H., Hosseini, S.H.R., Namihira, T., Katsuki, S., Akiyama, H. (2009) Fluid mechanics of torch appearance in capillary microplasma jet., Japanese Journal of Applied Physics 48 (1), no. 016001
  5. Fukuda, I., Ito, A., Hirai, G., Nishimura, S., Kawasaki, H., Saitoh, H., Kimura, K., Sodeoka, M., Yoshida, M. (2009) Ginkgolic acid inhibits protein SUMOylation by blocking formation of the E1-SUMO intermediate., Chemistry & Biology 16, 133-140.
  6. Saitoh, H., Uwada, J., Kawasaki, A. (2009) Strategies for the expression of SUMO-modified target proteins in Escherichia coli., Methods in Molecular Biology 497, 211-221.
  7. Takaki, K., Katsuki, S. (2009) Industrial Applications of Pulsed Power Technology, Electrical Engineering in Japan 129, pp.62-66.
  8. Choi, J., Wang, D., Namihira, T., Katsuki, S., Akiyama, H., Lin, X., Sato, H., Saeki, T. (2008) Inactivation of spores using pulsed electric field in a pressurized flow system., Journal of Applied Physics 104 (9), no. 094701
  9. Fudamoto, T., Namihira, T., Katsuki, S., Akiyama, H., Imakubo, T., Majima, T. (2008) Sterilization of E. coli by underwater pulsed streamer discharges in a continuous flow system., Electrical Engineering in Japan 164 (1), pp.1-7
  10. Garcia, M., Myouga, F., Takechi, K., Sato, H., Nabeshima, K., Nagata, N., Takio, S., Shinozaki, K., Takano, H. (2008) An Arabidopsis homolog of the bacterial peptidoglycan synthesis enzyme MurE has an essential role in chloroplast development. Plant J. 53, 924-934.
  11. Katsuki, S., Akiyama, H. (2008) Pulsed power driven discharge plasma EUV sources, Japan Society of Applied Physics 77 (4), pp.375-382
  12. Kawakami, T., Sakaguchi, K., Takechi, K., Takano, H. and Takio, S. (2008) Ammonium induced expression of the red algal chloroplast gene Ycf18, a putative homolog of the cyanobacterial NblA gene involved in nitrogen deficiency-induced phycobilisome degradation. Biosci. Biotechnol. Biochem. 73, 740-743.
  13. Lin, X., Minamisawa, N., Takechi, K., Zhang, W., Sato, H., Takio, S., Tsukaya, H. Takano, H. (2008) Isolation and characterization of the Larix gmelinii ANGUSTIFOLIA (LgAN) gene. Planta 228, 601-608.
  14. Peddigari, S., Zhang, W., Sakai, M., Takechi, K., Takano, H. and Takio, S. (2008) A copia-like retrotansposon gene encoding gypsy-like integrase in a red alga, Porphyra yezoensis. J. Mol. Evol. 66, 72-79.
  15. Peddigari, S., Zhang, W., Takechi, K., Takano, H. and Takio, S. (2008) Two different clades of copia-like retrotransposons in the red alga, Porphyra yezoensis. Gene 424, 153-158.
  16. Saitoh, H. (2008) Sumoylation., Experimental Medicine 56, 1089 [in Japanese].
  17. Sekiyama, N., Ikegami, T., Yamane, T., Ikeguchi, M., Uchimura, Y., Baba, D., Ariyoshi, M., Tochio, H., Saitoh, H., Shirakawa, M. (2008) Structure of the SUMO-interacting motif of MBD1-containing chromatin-asociated factor 1(MCAF1) bound to SUMO-2., J. Biol. Chem. 283, 35966-35975.
  18. Schoenbach, K., Kolb, J., Xiao, S., Katsuki, S., Minamitani, Y., Joshi, R. (2008) Electrical breakdown of water in microgaps., Plasma Sources Science and Technology 17 (2), no. 024010
  19. Uwada, J., Saitoh, N., Nakao, M., Saitoh, H. (2008) Modulation of Nuclear Structure and Cancer Development by the SUMO Modification System., Science of Human Body 59, 352-353 [in Japanese].
  20. Yasuoka, K., Maehara, T., Katsuki, S., Namihira, T., Kaneko, T., Hatakeyama, R. (2008) Generation of underwater plasmas and their characteristics, J. Plasma and Fusion Research 84 (10), pp.666-673
  21. Zhang, C., Cheng, Y., Katsuki, S., Akiyama, H. (2008) Dependence of extreme ultraviolet emission on xenon flow rate from a Z-pinch discharge plasma., Japanese Journal of Applied Physics 47 (6), pp.4878-4880
  22. Heeren, T., Camp, J., Kolb, J., Schoenbach, K., Katsuki, S., Akiyama, H., (2007) 250 kV Sub-nanosecond Pulse Generator with Adjustable Pulse-width, IEEE Transactions on Dielectrics and Electrical Insulation 14 (4), pp. 884-888
  23. Ihara, M., Koyama, H., Uchimura, Y., Saitoh, H., Kikuchi, A. (2007) Noncovalent Binding of Small Ubiquitin-related Modifier (SUMO) Protease to SUMO is Necessary for Enzymatic Activities and Cell Growth. J. Biol. Chem. 282, 16465-16475.
  24. Katsuki, S., Nomura, N, Koga, H., Akiyama, H., Uchida, I., Abe, S-I. (2007) Biological effects of narrow band pulsed electric fields, IEEE Transactions on Dielectrics and Electrical Insulation 14 (3), pp. 663-668
  25. Namihira, T., Sakai, S., Yamaguchi, T., Yamamoto, K., Yamada, C., Kiyan, T., Sakugawa, T., Katsuki, S., Akiyama, H. (2007) Electron temperature and electron density of underwater pulsed discharge plasma produced by solid-state pulsed-power generator, IEEE Transactions on Plasma Science 35 (3), pp.614-618.
  26. Nozaki, H., Takano, H., Misumi, O., Terasawa, K., Matuzaki, M., Maruyama, S., Nishida, K., Yagisawa, F., Yoshida, Y., Fujiwara, T., Takio, T., Tamura, K., Chung, S. J., Nakamura, S., Kuroiwa, H., Tanaka, K., Sato, N. and Kuroiwa, T. (2007) A 100%-complete sequence reveals unusually simple genomic features in the hot spring red alga Cyanidioschyzon merolae. BMC Biology 5:28.
  27. Ono, Y., Sakai, A., Takechi, K., Takio, S., Takusagaea, M. and Takano, H. (2007) NtPolI-like1 and NtPolI-like2, bacterial DNA polymerase I homologues isolated from BY-2 cultured tobacco cells, encode DNA polymerases engaged in DNA replication in both plastids and mitochondria. Plant Cell Physiol. 48, 1679-1692.
  28. Zhang, C.H., Katsuki, S., Shi, J.G., Horita, H., Namihira, T., Akiyama, H. (2007) Characterization of solid tin target for gas discharges produced EUV plasmas, Solid State Phenomena 121-123 (PART 2), pp. 885-888
  29. Zhang, W., Lin, X., peddigari, S., Takechi,, K., Takano, H. and Takio, S. (2007) Characterization of short interspersed elements (SINEs) in a red alga, Porphyra yezoensis. Biosci. Biotech. Biochem. 71, 618-622.